Enteroflor (intestinal microbiota of children 0-14 years old)
We remind you that independent interpretation of the results is unacceptable, the information provided below is for reference purposes only.
Enteroflor is a modern molecular test for intestinal microbiota assessment in children aged 0–14 years, based on quantitative PCR (qPCR) methods and microbial DNA detection.
The test evaluates the composition and balance of the intestinal microbiota, determines the quantitative and qualitative characteristics of major microbial groups, and identifies opportunistic and potentially pathogenic microorganisms.
The intestinal microbiota plays a key role in digestion, metabolism, vitamin synthesis, immune system development, and protection of the body. In childhood, its composition is actively developing and may be influenced by diet, infections, antibiotic therapy, and environmental factors.
Enteroflor is not a conventional stool culture but a highly sensitive molecular test that allows assessment of microbial balance at DNA level.
Indications
- functional digestive disorders (bloating, abdominal pain, colic)
- diarrhea or chronic constipation
- suspected intestinal dysbiosis
- food intolerances and allergic reactions
- decreased immune function
- recurrent infections
- inflammatory bowel diseases
- microbiota monitoring after antibiotic therapy
- comprehensive gastrointestinal assessment in children
Procedure
The test requires a stool sample collected after spontaneous bowel movement into a sterile container.
In the laboratory, molecular microbiota analysis is performed using microbial DNA detection methods, allowing evaluation of intestinal flora composition and balance.
Sample collection
- stool should be collected after spontaneous bowel movement, preferably in the morning
- the sample must be collected in a clean and dry container without contamination from urine, water, or detergents
- the sample is then transferred into a sterile container using a spatula
- a small amount should be taken from 3–5 different areas of the same stool sample (approximately 3 g)
- the container must be tightly closed and placed in a protective bag
- transport to the laboratory should be done as soon as possible, ideally within 2–4 hours
- until delivery, the sample should be stored at +2…+8 °C
Sources:
https://www.nature.com/articles/nrgastro.2017.97
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598837/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383038/
https://www.nature.com/articles/s41579-018-0029-9
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391518/
https://www.nature.com/articles/s44222-024-00168-3
https://www.frontiersin.org/articles/10.3389/fcimb.2020.00151/full
IMPORTANT!
It is crucial to remember that the information provided in this section is not intended for self-diagnosis or treatment. If you experience any symptoms or an exacerbation of a condition, it is essential to consult a healthcare professional for proper diagnostic testing and treatment. Only a qualified specialist can provide an accurate diagnosis and determine the appropriate course of treatment. To ensure the most accurate and consistent evaluation of test results, it is recommended to have them performed at the same laboratory. This is because different laboratories may use varying methods and units of measurement for similar tests.
Preparation:
- antibiotics should be discontinued at least 10 days before testing, ideally 2–4 weeks (as advised by a physician)
- probiotics, prebiotics, laxatives, rectal suppositories, and enemas should be avoided for 7–10 days before testing
- 24–48 hours before collection, avoid foods containing live bacterial cultures (yogurt, fermented dairy products, etc. )
- no special diet or fasting is required on the day of collection
- in case of recent acute gastroenteritis, testing should be performed 2–3 weeks after recovery